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The plates were briefly stirred and activity was spectrophotometrically determined (SpectraMax ABS Plus, Molecular Devices) at room temperature in kinetic mode at the specific wavelength for each substrate (described above)

Intact HCAEC (23105 cells/well) were incubated with 0500 M MGO for 1 h in serum-free medium, with enzymatic activities (measured as conversion of NADP+ to NADPH) determined with the following substrates after lysis: (A) glucose 6-phosphate, G6P

4.1 Catalase Catalase (H 2 O 2 :H 2 O 2 oxidoreductase, EC 1.11.1.6) is an intracellular enzyme found at high concentrations in erythrocytes and liver peroxisomes in mammals [48, 49, 50, 51]

While gluten-free foods can be made using naturally gluten-free grains such as rice and millet, scientists have mixed the enzymes into wheat, barley, and rye-based flour, where they can easily access and break down gluten

G., Boos, N., Wiest, I