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To validate these hypotheses, further experiments are necessary

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In PAM212 cells, 12-hour closure was lower but still notable, achieving 87% (GHK-AgNPs) and 90% (GHK-Cu-AgNPs) at 24 hours, both significantly higher than controls

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The stirring continued for another 12 h at room temperature, after which the mixture was transferred into 10 mL of deionized water while being sonicated