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Frequently Asked Questions Why is the LR3 variant used instead of native IGF-1 in research

The top 5 precursor ions were subsequently fragmented, in a data-dependent manner, using the higher energy collisional dissociation (HCD) cell set to 30% normalized collision energy in MS2 at a resolution power of 17,500

The HRP reaction was terminated by addition of 1 M HCl and the 450 nM (HRP) or 405 nm (AP) absorption values was recorded using a Sunrise Spectrophotometer (TECAN)