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51,52 Mtb infection activates both of these NLRs in an ESX-1-dependent manner to trigger various host downstream innate immune responses, such as NLRP3 inflammasome formation, autophagy initiation and NF-B and IRF3 pathway activation, which have been extensively reviewed elsewhere

Gene expression analysis Total RNA was extracted from cells or tissues using Trizol (Thermo Fisher Scientific) or the RNeasy kit (QIAGEN)

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JR: Methodology (supporting), Supervision (equal), Writing-reviewing and editing (equal), Resources (equal)

[25] Alternativamente la actividad puede medirse por medio de roGFP ( redox-sensitive Green Fluorescent Protein es decir protena verde fluorescente sensible al estado redox)