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The qPCR was performed using PerfeCTa SYBR Green SuperMix (Quantabio) conducted on an ABI StepOne Real-Time System following the cycles: 95 C for 3 min and 40 cycles of 95 C for 10 s and 60 C for 30 s

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The diagnosis requires a depressed mood most of the day, more days than not, for at least two years in adults (one year in children and adolescents)

Exercise: Alcohol may be eliminated slightly faster during exercise

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