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In particular, the use of desthiobiotin allows elution under near-physiological, neutral pH conditions, thereby minimizing the risk of acid- or chemical-induced protein denaturation [124]

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### Step 7: Inspect the Solution The reconstituted solution should be: - Clear (not cloudy or hazy) - Colorless (no yellow or brown tint) - Free of visible particles or floaters - Completely dissolved -- no powder clinging to the vial walls **If any of these criteria are not met, do not inject.** Cloudiness or particles indicate degradation or contamination

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