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Thus, TNBS promotes two independent mechanisms of injury, GSH depletion and ROS generation, both being required for the manifestation of mucosal injury as GSH limitation renders intestine susceptible to the TNBS-induced ROS overgeneration

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1209 These findings indicate that the combination of CRISPR-Cas9 gene editing technology with single-cell metabolomics and human organoids holds promise for accurately monitoring cellular metabolic changes in relevant diseases

Biochem Res Int 2016:2645237