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Since there is no subsequent time monitoring, the expression changes are not clear

doi: 10.3390/nu11071663

PMID: 14763161

Shim M, Shi Kam NW, Chen RJ, Li Y, Dai H

To collect BM extracellular fluid, femurs and tibiae were flushed with 1 mL of ice-cold PBS, and the supernatant was collected after centrifugation at 500 g for 5 min at 4 C