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Six hours after the addition of labelled media, a subset of wells was harvested by scraping in PBS and centrifugation (300 g for 5 min)

It also helps your body to use fat and carbohydrates for energy and make new proteins

Lpez-Bermejo A, Khosravi J, Corless CL et al (2003) Generation of anti-insulin-like growth factor-binding protein-related protein 1 (IGFBP-rP1/MAC25) monoclonal antibodies and immunoassay: quantification of IGFBP-rP1 in human serum and distribution in human fluids and tissues