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Deletions and point mutations were introduced to into the plasmid using the primers presented in Supplementary Table 1 with the Q5 Site-Directed Mutagenesis kit (New England Biolabs) according to the manufacturers instructions

A splicing mutation affecting expression of ataxia-telangiectasia and Rad3-related protein (ATR) results in Seckel syndrome

Tretinoin use can potentially be very irritating, so it has not been deemed safe enough [by the FDA] at this point to be available over the counter, says Hadley King, MD, a New York-based board-certified dermatologist