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Description

The GFP thermal shift assay for screening ligand and lipid interactions to solute carrier transporters

Nonetheless, household exposure is probably more relevant than public exposure, and the continued declines in smoking rates as well as health regulations outlawing smoking in indoor public spaces will further mitigate the effects of SHS

Response varies with the severity and chronicity of the injury

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Briefly, the cells were lysed with RIPA buffer (Sigma Aldrich) containing 150 mM NaCl, 1.0% Nonidet-P 40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 50 mM Tris (pH 8.0), a protease inhibitor cocktail, and PhoSTOP (Roche Molecular Biochemicals, Basel, Switzerland)