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The membrane was incubated with 5% (w/v) non-fat dried milk as a blocking solution and then probed with the indicated primary antibodies (Merck, USA), including anti-iNOS (1:2000) and anti--actin (1:2000)

3, 10, 11, 13, 15, 16, 20, 45, 47, 48 Two of these studies did not include a healthy comparison group 13, 45 and one did not report group means or effect sizes for Glx, 48 but for the remaining seven studies, we calculated the effect size (Cohens D) from the difference between group means (ADHDcontrol) and the pooled standard deviations for Glx/Cr or Glx

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Various pathways digest nutrients in the gut