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For the PPO activity assay, 3 mL of reaction solutions, consisting of 2.7 mL 0.1 mol L 1 PBS, 0.1 mL catechol, 0.2 mL crude enzyme solution

Am J Phys Cell Physiol 308:C827C834 Salas-Burgos A, Iserovich P, Zuniga F, Vera JC, Fischbarg J (2004) Predicting the three-dimensional structure of the human facilitative glucose transporter glut1 by a novel evolutionary homology strategy: insights on the molecular mechanism of substrate migration, and binding sites for glucose and inhibitory molecules