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To verify that the SAT1 disruption cassettes were successfully excised from GDH2/gdh2 and GDH3/gdh3 strains, genomic PCR was used with primers C1 and C4 to amplify the region between the upper end of 5 flank sequences to the lower end of 3 flank sequences to make sure that both alleles of each gene were removed (Supplementary Fig

Riddle DL, Blumenthal T, Meyer BJ, Priess JR (1997) Introduction to C

Given our in vitro evidence that LCN2 promotes osteocyte ferroptosis, we next examined whether Lcn2 ablation in osteocytes alters expression of ferroptosis-related genes (FRGs) in vivo

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