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After TMB substrate solution is added, only those wells that contain Human IFNg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

Inflammation and depression have a bilateral relationship, as depression enhances the activation of CRP, IL-1, and IL-6, which is positively associated with inflammation and increased BMI and vice versa [134]

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Also, make a list of any health conditions you have, especially those related to weight, like high blood pressure or diabetes

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