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The cell lysate was then subjected to centrifugation at 27,000 g for 40 min, followed by ultracentrifugation at 220,000 g for 1 h at 4 C to remove the cell debris and the membrane fraction from the lysis solution

In experimental systems, the complex provides a controlled way to study copper-linked changes in collagen-associated pathways, inflammatory mediators, and oxidative stress markers

These antibodies were tested for in vivo protection, structural analysis and binding and neutralization across diverse sarbecovirus and VoC to further elucidate the molecular mechanism of broad antibody recognition following SpFN vaccination