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Cell lysates were cleared by centrifugation at 16,000 g for 30 min at 4 C

This approach makes sense, and since thiamine is water-soluble, it is extraordinarily safe to do and poses very little risk of toxicity

then, the solvent was dried under a nitrogen flux for 25 min, and the tubes were placed in a vacuum chamber for 40 min to remove the remaining solvent

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References Patti, G