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To construct the modified homozygous strain expressing GAP1 as the counter-selectable marker in the BY4743 background, the KanMX fragment was amplified from pFA6A-KanMX6 [32], and HS fragments for pGAP1 were amplified from yeast genomic DNA and these fragments plus pGSH1 (above) assembled into pRS315 to give pRS315- HS-KanMX-pGSH1-HS

Myeloid cells, including macrophages and neutrophils, are especially implicated in the respiratory burst

doi:10.1016/j.numecd.2022.05.008 Jerneren F, Cederholm T, Refsum H, et al

Various factors, including genetic predisposition, immune dysregulation, and alterations in the gut microbiota, are believed to contribute to the pathogenesis of UC

Borhani, S., Borhani, R