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Finally, alterations in the NAD+/sirtuin pathway may induce aging via the p53/p21 pathway but could also negatively affect the specific functions of forkhead box O (FOXO) and peroxisome proliferator-activated receptor coactivator 1 (PGC-1), leading to increased ROS and mitochondrial dysfunction 55

5 and 6, samples in SDS solubilizer (0.0625 M TrisHCl (pH 6.8), 10% glycerol, 0.01% bromophenol blue, 2% (wt/vol) SDS, +/- 2% 2-mercaptoethanol) were heated at 95 C for 5 min, electrophoresed through 8% or 10% (wt/vol) polyacrylamide-SDS gels, transferred to nitrocellulose membranes (Bio-Rad, USA), and incubated with SARS-CoV-2 (2019-nCoV) S antibody rabbit PAb antigen affinity purified, mouse monoclonal anti-SARS-S2 conjugated to HRP, goat anti-human IgG-HRP, ACE2 recombinant rabbit monoclonal antibody, or purified LgBiT-substrate cocktail

Biomaterials 29, 29963003

J Plant Nutr 42(9):10011015

10.1016/s0891-5849(01)00480-4 183 SchieberM.ChandelN