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The effector and target cells were cocultured for 15 h at a 1:1 ratio (1 10 5 cells) in 96-well plates, and then, cell death was measured using the FITC Annexin V Apoptosis Detection Kit I (BD Biosciences)

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5,8,21 Chronic alcoholism and APAP overdose may potentiate liver failure, but there is no indication that the combination of alcoholism and taking therapeutic amounts of APAP will necessarily cause hepatotoxicity