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The inflammatory response is further exacerbated in lung injury, leading to diffuse damage to alveolar epithelial cells, decrease in lung surface-active substances, destruction of the alveolar walls, increased permeability of the basolateral membranes, accumulation of intra-alveolar fluid, accumulation of polymorphonuclear leukocytes, parenchymal cell damage, and interstitial edema (Meng et al., 2018)

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The medium was supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% Penicillin-Streptomycin (P/S)