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As cancer cells are in a persistent state of oxidative stress with high levels of ROS production, they have a low tolerance thresholds for additional ROS and activation of cell death pathways 17

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For example, methapyriline increases liver weight and the number of hepatocyte mitochondria in treated mice, with development of hepatotoxicity and liver neoplasia probably secondary to oxidative damage (Powell et al

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We then tested ATP abundance by liquid chromatographymass spectrometry (LCMS) and show increased ATP levels in cells that survive 5FU treatment (Fig