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Moreover, by immunostaining and expression analysis of primary endothelial cells, we observed reduced protein and mRNA abundance of the tight junction protein occludin, and reduced Abcb1a mRNA of the ABCB1 (P-glycoprotein) efflux transporter, likely explaining the decreased tightness of the barrier in vitro (Fig

For all constructs, except for the non-binding control NAPstarC and NAPstar4, we observed an NADPH concentration-dependent change in the TS/mC fluorescence emission ratio ( Figure 1C )

Siegel MP, Kruse SE, Percival JM, Goh J, White CC, Hopkins HC, Kavanagh TJ, Szeto HH, Rabinovitch PS, Marcinek DJ

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followed by, One cycle of 72 C for 5 min, finally held at 4 C, a 100 bp DNA ladder (GeneDirex, Cat No