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The supernatants were collected by centrifugation at 5000 rpm for 5 min at 4 C and precleared by incubated with GFP-Trap agarose beads (Chromotek) or HA-Beads (Roche) for 2 h at 4 C with rotation

Prediction analyses were performed through backward stepwise Cox proportional hazards regression and expressed as the hazard ratio (HR)

Chousterman, B

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