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The variable importance in the projection (VIP) values from OPLS-DA models, fold change (FC), and p -value or false discovery rate (FDR) correction were performed to screen the differential metabolites

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For ultra-performance liquid chromatographytriple quadrupole mass spectrometry (UPLCTQMS), the metabolite extract was diluted with 20% (v/v) acetonitrile

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The iPSC-derived heart grafts displayed normal beating for a duration of over 3 months in syngeneic recipients, demonstrating their functional viability and long-term survival