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The PCR product was subjected to restriction digestion with 10 U of the Bss T1I enzyme (Sibenzyme) at 60 C for 4 h followed by 2.5%-agarose gel electrophoresis

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The Association Syndrome de Wolfram ( organizes a family day so that families can get together and share their experiences in coping with the disease

This balance between size and specificity is frequently cited as a design achievement of the original Monash research program and is one reason the molecule has endured as a reference compound across multiple laboratories