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CCK-8 results were similar to those observed with RAW264.7 macrophages, with cell death observed in all five cell types (BMDM, THP-1, U937, A549, and BEAS-2B), with a much higher death rate of epithelial cells (A549 and BEAS-2B) (Fig

Quantification was done in at least 10 fields for each condition and the proportion of dead cells was calculated as a percentage of total cells expressing the WT or WD-associated variants

Nature 575 , 217223 (2019)

Much like all skin concerns relating to the eye area (like under-eye milia, for example), dark circles are stubborn and they can crop up for a whole host of reasons