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Oxidants in chronic kidney disease

It was reported that knockdown of NAD + kinase (NADK), an enzyme that uses NAD + to synthesize NADP(H), was able to decrease NADP(H) levels in HT1080 cells and make them more susceptible to ferroptosis inducers (Shimada et al., 2016a)

The idea behind step gradients is to control individual compound elution as much as possible in an effort to maximize the separation between compounds