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Companion satellite-cell cultures, prepared at the same time, were immunostained at 30-h post-plating with D3 anti-desmin mAb and DAB (a chromogenic HRP-substrate that forms an insoluble brown reaction product) to determine the percentage of myogenic cells present 18 ,19 cultures with less than 95% DAB-positive cells (namely, less than 95% desmin-positive myogenic purity) were not used for experiments just to ensure the reliability of data specific to myogenic cells in the employed primary cell culture systems

The ASTN2 gene has been implicated in ASD, and genome-wide association studies have shown that ASTN2 is a candidate gene for ASD [95]

A full characterization was conducted for dual PAM JPC0323 (Figure 6), which evoked a 44% increase in maximum 5-HT-induced Ca2+ intake and also showed negligible displacement at orthosteric binding sites of a number of GPCRs and transporters and exhibited favorable pharmacokinetic parameters

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This dysbiosis can produce metabolites that influence neurotrophic factor signaling pathways in the brain (Maqsood and Stone 2016)