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Although GHRH-R couples with the G s protein, the addition of MK-0677 to rat primary cultured pituitary cells increased intracellular Ca 2+ levels, suggesting the existence of a second G protein-coupled receptor (GPCR) that couples with the G q protein, thereby promoting GH secretion through a pathway distinct from that of GHRH-R (Patchett et al., 1995)

Current Opinion in Pharmacology

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Embryonic Stem Cells (ESCs) For generation of Ube3C knock-out mice, mutant JM8A3.N1 ES cells were purchased from EUCOMM Consortium (Ube3C tm1a(EUCOMMHmgu) )