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Role of autophagy in temozolomide-induced cytotoxicity for malignant glioma cells

C2C12 myotube signaling C2C12 myoblasts (CRL-1772, ATCC) were seeded at near-confluence in 12-well tissue culture dishes and allowed to adhere overnight in growth medium (DMEM + 10% FBS + 1% ABAM)

The repeated finding that E-domain effects are strongest in young, growing tissue and blunted with age seen both in cultured human satellite cells [6] and in virally treated mouse muscle [21] is a particularly pointed reminder that the models most likely to show a positive result are the least representative of the adult humans who feature in performance narratives

Baran P, Hansen S, Waetzig GH, Akbarzadeh M, Lamertz L, Huber HJ, et al