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The cell pellets were resuspended in lysis buffer (50 mM HEPES, pH = 7.5, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.5% NP-40, 0.25% Triton X-100 with protease inhibitors), incubated on nutator for 10 min at 4 C and centrifuged at 500 g for 5 min

Low Se concentration may increase sperm susceptibility to free radicals, which may disturb the biochemical processes taking place in the acrosome [138]

This pricing mechanism is designed to satisfy the need for fair reimbursement to providers while also managing the overall cost to the Medicare program

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J Toxicol Clin Toxicol 40:320 Schidt FV, Rochling FA, Casey DL, Lee WM (1997) Acetaminophen toxicity in an urban county hospital