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50 g of total protein was incubated in 1 ml GST reaction buffer (100 mM potassium phosphate pH 7.8, 1 mM CDNB in 80% ethanol) and the reaction was started by adding 100 l of 10 mM GSH

However, injections of 55 or 75 mg/kg/day of EGCG induced hepatotoxicity in mice, accompanied by inhibition of hepatic antioxidant enzymes and increased nuclear distribution of Nrf2 (231)