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Beads were then washed twice with 5 ml of equilibration buffer and twice with 5 ml of capture-wash buffer (18 mM HEPES pH 7.4, 2 M NaCl, 1 mM MgCl 2 , 10% (v/v) glycerol, 0.003% (v/v) IGEPAL CA-630, 0.033 mg ml 1 recombinant albumin and 0.005 mg ml 1 yeast tRNA), then incubated with 1 nmol 3-biotinylated DNA competitor oligonucleotide (IDT) complementary to the capture oligonucleotide (Supplementary Table 4) in 18 mM HEPES pH 7.4, 1 M NaCl, 1 mM MgCl 2 , 10% (v/v) glycerol, 0.003% (v/v) IGEPAL CA-630, 0.033 mg ml 1 recombinant albumin and 0.005 mg ml 1 yeast tRNA, at 25 C for 2 h with end-over-end rotation

tarentolae cells as well as a mCherry gene enabling monitoring of expression after induction

In general, diverse oxidative stress responses were identified, indicating that the DW biofilm responded to residual disinfectants by activating multiple antioxidant systems rather than depending on a single mechanism for protection 41

Besides, hydrophobic interactions, hydrogen bonds, electrostatic interactions, etc., are the driving forces for polymer micelle formation [132]