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Li SR, Man QW, Gao X, Lin H, Wang J, Su FC, et al

Five random fields per ibidi well/coverslip were scanned using a 10x lens of a Leica fluorescence microscope (DM6000), and the total number of cells, the number of cells labeled with each antibody, and the total number of MBP + /Olig2 + cells or MBP /Olig2 + cells per field were counted with ImageJ and compared

The study revealed alterations in insulin signaling pathways, the cell cycle, cellular senescence, inflammatory mediators, and mitochondrial respiratory electron transport chain components (58)