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Taking into account all the published data of these studies, we noted a significant reduction in the ORR of patients undergoing combination (76) ( Figure 5 )

Cell migration and invasion assay Cell migration and invasion assays were determined using 24-well transwell inserts or Matrigel transwell inserts from BD Biosciences (San Jose, CA, USA), respectively as previously described with modification

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Specifically, SIRT1, SIRT3, and SIRT5 protect the cell from ROS, and SIRT2, SIRT6, and SIRT7 modulate oxidative stress 138,139