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The 2006 Ionescu paper documented that pulsatile GH secretion persisted under continuous CJC-1295 stimulation, an unexpected finding that has shaped subsequent GH-axis research interpretation (PMID 17018654)

Injecting more frequently than every 3 hours produces diminishing returns because the somatotroph cells have not had time to resynthesize and store growth hormone for the next pulse

So when the peptide is injected subcutaneously, it finds albumin in the interstitium and plasma and forms a covalent bond to Cys34

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