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After washing three times for 15 min each with 0.3% Triton X-100 in PBS and three times for 1 min each with PBS, the optical clearing started by incubating the slices in 25% fructose for 4 h, continued in 50% fructose for 4 h, 70% fructose overnight, 100% fructose (100% wt/vol fructose, 0.5% 1-thioglycerol and 0.1 M phosphate buffer, pH 7.5) overnight, followed by a final overnight incubation in SeeDB solution (80.2% (wt/wt) fructose, 0.5% 1-thioglycerol and 0.1 M phosphate buffer) 63

The timelines are different too

These compounds differ in how they work, what they target, how long they stay active in the body, and what evidence exists to support their use

doi:10.2147/ijn.S241055 184

h , i , Immunoblot of mouse XKR8 mutants (D30A and W45A, Flag-tagged) in HL-60 XKR8 KO cells