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Measurement of glutathione peroxidase and glutathione reductase activity To measure GPx activity, 120L of saliva were incubated in a final volume of 3mL with phosphate-EDTA buffer (100mM, pH 7.5, EDTA-Na2 1mM) in the presence of 30L of NADPH (20mM), 100L of GSH (60mM), and 4L (1IU) of GRd for 5min at room temperature

The CareStart G6PD Biosensor 1 (Wells Bio, Seoul, Republic of Korea), a newer generation and more quantitative method of testing, is also reportedly more sensitive (72)