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The study utilized partial least square discriminant analysis and found increased risk associations between the metabolites ornithine, 1-methylhistidine, methyl jasmonate, benzoate, nonanioic acid and 10-hydroxydecanoate with ASD diagnosis (statistical quantification not specified)

Real-time-qPCR samples were preheated at 95C for 10 min, followed by 40 cycles of denaturation at 95C for 15 s and annealing/extension at 60C for 1 min