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ELISA assays complement the HPLC analysis by measuring concentrations of melatonin and neurotrophic factors, utilizing kits from Sangon Biotechnology Company (Shanghai, China) following strict protocols to ensure reliability

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Research published by Pickart, Downey, and colleagues showed that GHK-Cu-treated wounds in rodent models exhibited increased collagen synthesis and more organised extracellular matrix architecture compared to controls

Research models frequently examine GHKCus role in metalion transport , ECMassociated signaling , redoxbalance pathways , and cellularresponse mechanisms , making it a widely studied tool in metallopeptide and oxidativestress research

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