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Cell fractionation Cells were resuspended in fractionation buffer (250 mM sucrose, 20 mM Hepes, 10 mM KCl, 1.5 mM MgCl 2 , 1 mM EGTA, 1 mM EDTA, 1X protease inhibitor cocktail) and then sonicated (4 15 s, 50% amplitude)