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Count the cells to ensure they are at the proper concentration

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The beads were packed into a gravity column, and washed with 15 CV of buffer with 10 mM imidazole in buffer 0.03%(w/v) DDM, 0.006%(w/v) CHS, 1 M NaCl, 4%(w/v) glycerol, 0.05 mM EDTA, 0.2 mM TCEP, 0.1 protease inhibitor cocktail, 20 mM HEPES pH 7.5