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Construction of NQO1 mutant cell lines HEK293T cells were transfected with a plasmid encoding Cas9-NLS, an NQO1-targeted sgRNA (target region, 5-CCATCTGAGCCCAGATATTG-3), and each of the HDR templates (S82D, GCTTATAAAGAAGGCCATCTGGACCCAGATATCGTTGCTGAACAAAAGAAG

Primary outcomes included modified Rankin Scale (mRS) scores at 90 days for functional outcomes: excellent (mRS 01), good (mRS 02), and poor (mRS 35)

Induction of Ferroptosis by Shikonin in Gastric Cancer via the DLEU1/mTOR/GPX4 Axis

The exact reconstitution calculations

Dexamethasone reduces food intake, weight gain and the hypothalamic 5-HT concentration and increases plasma leptin in rats