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Finally, 10 L of the reconstituted samples were run on Hypersil Gold C8 column (100 2.1 mm) with 0.1% formic acid in water as mobile phase A and 0.1% formic acid in acetonitrile as mobile phase B on SCIEX 6500 + QTRAP, and peak retention time was matched with standard (Methionine) across all the samples

Methyl alcohol was supplied by El-Gomhoria Company, Cairo, Egypt

Feng Q, Yang Y, Qiao Y, Zheng Y, Yu X, Liu F, et al

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In the FY 2020 IPPS/LTCH PPS final rule (84 FR 42308), we modified our methodology with regard to how dollar amounts, hours, and other numerical values in the unadjusted and adjusted wage index calculation are rounded, in order to ensure consistency in the calculation