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Measurement of the concentration of macro and micro elements The wet digestion method was used to measure the concentration of macro and microelements 27 for which 500 mg of dry and ground leaf samples were digested in a mixture of sulfuric acid (H 2 SO 4 ) and nitric acid (HNO 3 ) at a ratio of 1:5 (v/v) at 60 C for 24 h

In particular, miR-122-5p, which is already established as a critical hepatic biomarker, was upregulated in MASLD serum, consistent with studies in adults

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Each segments immunoreactivity was examined using a (x 400) high-power microscope in 10 microscopical areas