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Materials and methods Post-mortem brain samples Frozen post-mortem tissues were obtained from the NICHD Brain and Tissue Bank for Developmental Disorders at the University of Maryland, Baltimore, MD, USA, and from the Autism Tissue Program at the Harvard Brain Tissue Resource Center, Belmont, MA, USA

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Metabolite extraction from serum and tissues For metabolomics analysis, 500 L of extraction solvent (ACN: MeOH: H 2 O = 2:2:1, v/v/v) was added to 100 L of serum or 25 mg of tissue sample

IV Skinny Drip L-Carnitine An infusion combining amino acids, electrolytes, and selected vitamins

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