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The column was washed with 6 bed volumes of wash buffer (50 mM sodium phosphate, 300 mM NaCl and 100 mM imidazole, pH 7.4) and the target protein was eluted with 6 bed volumes of elution buffer (50 mM sodium phosphate, 300 mM NaCl and 300 mM imidazole, pH 7.4)

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Additionally, in vitro experimentations have established that papain, a plant proteolytic enzyme mined from papaya, is proficient in altering how immune cells persuade an inflammatory reaction by constraining their fabrication of main inflammatory mediators

Diarylheptanoids and galangin were suggested to be mainly responsible for the antiproliferative activity of the plant toward human glioblastoma cancer cells (Liu et al., 2014)

Bowers R, Cool C, Murphy RC, Tuder RM, Hopken MW, Flores SC, Voelkel NF