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10.1111/exd.13548 5 Carvajal-YepesM.HimmelsbachK.SchaedlerS.PloenD.KrauseJ.LudwigL.et al (2011)

The Science Behind Fat Metabolism At the cellular level, fat metabolism is a multi-step process that converts stored fat into usable energy

sgRNA constructs and gene expressing codon optimized (OPT) plasmid constructs sgRNA control and constructs against CBS and L2HGDH were designed targeting GTATTACTGATATTGGTGGG (sgControl) (#230080, Addgene), GGAGCAGACAACCTACGAGG (sg CBS -1) (#230081, Addgene), TGGCAGTGCTGGCAGCACGG (sg CBS -2) (#230082, Addgene), GATATAGTCATCGTTGGTGG (sg L2HGDH -1) (#230083, Addgene), and CACCAGACTGGACATAACAG (sg L2HGDH -2) (#230084, Addgene) and constructed in lentiCRISPR v2-Blast (#83480, Addgene) for Blasticidin selection at a final concentration of 5 g/ml

doi: 10.1016/j.jbior.2020.100741 60 SestiliP.StocchiV

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