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In cultured dermal fibroblasts, assays quantify pro-collagen I/III and matrix metalloproteinases (MMP-1, MMP-9) alongside their inhibitors (TIMPs)

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Another study with CuCl 2 treated Hep G2 cell line demonstrated that mitochondrial toxicity is an intermediate-level event associated with copper toxicity to Hep G2 cells while lysosomal damage is the early event and plasma damage a late feature associated with copper toxicity 34

Induction of an aging mRNA retinal pigment epithelial cell phenotype by matrix-containing advanced glycation end products in vitro